《植物生理学报》 2014, 50(8): 1177-1183
通信作者：段晓宇；E-mail: dxy861179@163.com；Tel: 18583910058
摘 要：
采用自然降温处理, 研究稀土镧(LaCl3)对大花蕙兰组培苗耐寒性的影响, 确定培养基中加入La3+的浓度, 比较地上部和地下部在反映组培苗耐寒性能方面的差异性。分别测定了地上部和地下部中丙二醛(MDA)含量、膜透性、可溶性蛋白(SP)、可溶性糖(SS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT) 7项与耐寒性有关的生理指标。结果表明, La3+提高大花蕙兰组培苗耐寒性的最适宜浓度为20 mg•L-1, 浓度过高(60 mg•L-1)出现负效应。膜脂过氧化产物丙二醛(MDA)含量和细胞膜透性两项指标在反应大花蕙兰组培苗耐寒性作用明显, 可作为其耐寒性鉴定指标。各浓度LaCl3处理对大花蕙兰组培苗耐寒性的提高差异明显, LaCl3对地上部和地下部耐寒性的提高在不同指标间表现出不一致性。关键词：LaCl3; 大花蕙兰组培苗; 耐寒性; 鉴定指标
收稿：2014-03-18 修定：2014-07-17
Corresponding author: DUAN Xiao-Yu; E-mail: dxy861179@163.com; Tel: 18583910058
Abstract:
To determine the concentrations of lanthanum chloride (LaCl3) in medium and compare the difference between leaf and root in cold resistance of Cymbidium plantlets in vitro, the effects of lanthanum ions on cold resistance of plantlets based on natural cooling treatment were studied. Seven indexes related to cold resistance including MDA content, membrane permeability, soluble protein content, soluble sugar content, SOD activity, POD activity and CAT activity of leaf and root were determined. The results showed that the optimum concentration of LaCl3 to improve cold resistance of C. hybridum plantlets in vitro was 20 mg•L-1. High concentrations of LaCl3 (60 mg•L-1) had negative effects on the cold resistance. MDA and membrane permeability significantly reflected the cold resistance and could be the indexes in Cymbidium plantlets in vitro. Each concentration of LaCl3 improved the cold-resistance differently. The coldresistance improvement of LaCl3 on aboveground and underground showed inconsistency between different indexes.Key words: LaCl3; Cymbidium plantlets in vitro; cold resistance; identification index
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